High-throughput screening of surface markers on bone marrow-derived hematopoietic lineage cell populations from up to 25 donors simultaneously

Immunophenotyping panel
Conclusion:
Presented panel allows simultaneous analysis of almost all nucleated cells of hematopoietic origin from up to 25 donors simultaneously. The usage of a combination of CD45 and CD71 for sample barcoding overcomes the limitation of previously used barcoding techniques and allows simultaneous analysis of changes in immune cells and changes in erythropoiesis, which are often closely related. Finally, this is the first panel that combines barcoding of samples using abundant surface molecules with over 40-parameters spectral flow cytometry and in combination with commercially available kits of PE-labelled antibodies, such as LegendScreen kit from Biolegend, allows screening of several hundred surface markers on the nucleated cells of hematopoietic origin from up to 25 donors simultaneously.