Impact of Insulin-like growth factor-1 (IGF-1) on the aspect ratio and gene expression of rabbit Achilles tenocytes

We present data on the aspect ratio of rabbit Achilles tenocytes in vitro, based on measuring width and length of the cells, when stimulated with 1, 10 or 100 ng/mL IGF-1 supplementation to culture medium against corresponding cell cultures without IGF-1 supplementation for 3 days.

Furthermore, we present for two rabbit donors the impact of 0.1, 1 and 10 ng/mL IGF-1 supplementation to Achilles tenocytes in vitro and of 1 ng/mL IGF-1 that was released from an emulsion electrospun mesh with incorporated IGF-1 to test if the released IGF-1 was still bioactive. Gene expression of target genes collagen I, ki67 and tenomodulin are presented; the data were normalized to gene expression of corresponding cell culture without IGF-1 supplementation.