In vivo transcriptome analysis provides insights into host-dependent expression of virulence factors by Yersinia entomophaga MH96, during infection of Galleria mellonella

Genome-wide transcriptional responses of Yersinia entomophaga to shifts in temperature (25 - 37 degrees C) and in vitro and in vivo growth conditions were investigated using RNA-seq. Overall design: In vivo samples included four biological replicates and in vitro samples included two biological replicates. At 25 degrees, lag, exponential and stationary growth stages were sampled from in vitro and in vivo conditions. At 37 degrees only exponential growth conditions were sampled.