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SCX, 18O-labeling and charge reversal for C-terminomics

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NIAID Data Ecosystem2026-03-11 收录
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https://www.omicsdi.org/dataset/pride/PXD013486
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资源简介:
The proteome wide, mass spectrometry based identification of protein C-termini is hampered by factors such as poor ionization efficiencies, low yields in labeling strategies or the need for enrichment procedures. We present a bottom-up proteomics workflow to identify protein C-termini utilizing a combination of strong cation exchange chromatography, on-solid phase charge-reversal derivatization and LC-MS/MS analysis.
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2019-06-20
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