Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

This is a microarray experiment to investigate changes in gene expression in deseeded berries from field grown vines to identify genes involved in diurnal and circadian effects. Changes in gene expression at two time points within a 24 hour cycle were analyzed to identify expression profiles that coincided with diurnal and circadian effects. The gene expression profiles were then to be compared between the two developmental time points T5 v T17). Keywords: Diurnal v Circadian Overall design: Two time points during the diurnal cycle were analyzed at preveraison and post veraison for gene expression changes caused by diurnal or circadian rhythms using the Affymetrix one colour array platform. Two biological replicates were collected for each developmental stage. Biotin labeled probes were prepared from the extracted RNA from each of the pooled berry samples and hybridized to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridization station was randomized using the AlgDesign package from R.