Knocking down the eIF4E gene in HaCaT cells, which M5 stimulates to establish the in vitro psoriasis cell model.. Knocking down the eIF4E gene in HaCaT cells, which M5 stimulates to establish the in vitro psoriasis cell model.

We transfected keratinocytes cell line (HaCaT cells) with si-NC and si-eIF4E, and added M5 into the cell culture medium to reveal the function of eIF4E in the in vitro psoriasis cell model. Overall design: We set up si-NC+M5 (n=3) and si-eIF4E+M5 groups (n=3) in HaCaT cells, extracting total RNA after 48 hours, and sent the obtained samples for transcriptome sequencing to reveal the function of eIF4E in psoriatic keratinocytes.