10X single-cell RNA-sequencing profiling of hepatic non-parenchymal cells from LPS or PBS-administrated Hlj1 knockout and littermate wild-type mice.

Hlj1 knockout mice survive better than wild-type mice when injected with a lethal dose of lipopolysaccharide (LPS). As the liver is the major source for production of inflammatory cytokines and is also responsible for bacterial and toxin clearance when hosts are exposed to pathogens, we asked whether Hlj1 participated in immune modulation in the liver. We isolated and sequenced each hepatic non-parenchymal cells from these mice, and identified Hlj1 as the key regulator to enhance IFN-g-related signaling in NK cells. Hlj1 wild-type (WT) and Hlj1 whole-body knockout (KO) mice are intraperitoneally injected with 20 mg/kg LPS or PBS as control and sacrificed 8 hours post injection. Largest liver lobe of n=3 mice from same group are pooled together and grinded to isolate and purify non-parenchymal cells, which were prepared for sc-RNA sequencing using the 10X chromium platform.