FLAG-IP–MS profiling of SPINDOC interactome using a HeLa–Tet-On–FLAG-SPINDOC-GFP cell line
收藏国家人口健康科学数据中心2026-06-06 收录
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HeLa–Tet-On–FLAG-SPINDOC-GFP cells were established for inducible expression and affinity purification. FLAG-SPINDOC-GFP expression was first induced by doxycycline treatment. Subsequently, cells were either left unsynchronized or arrested at the G2/M phase by nocodazole treatment. Efficient expression and enrichment of FLAG-SPINDOC-GFP were confirmed by FLAG co-immunoprecipitation. Immunoprecipitates were then subjected to mass spectrometry analysis to identify SPINDOC-associated proteins, particularly under mitotic conditions.
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北京蛋白质组研究中心创建时间：
2026-05-15
