Effects of Voluntary Running Wheel Exercise-induced Extracellular Vesicles on Anxiety

Exercise induces the release of extracellular vesicles (EVs) into the circulation, which transmit signals between organs. EVs contain microRNAs (miRNAs) that inhibit mRNA translation or enhance mRNA degradation. However, the effects of exercise-induced EVs on anxiety remain poorly understood. We used microarrays to figure out the global alteration of gene expression in the hippocampus underlying stress and sedentary or exercise-induced EVs. We isolated EVs from the sera of mice that were sedentary or that voluntarily exercised. After then, we analyzed changes in mice with 3 weeks of restraint stress-induced anxiety after injection of 20μg amounts of EVs from exercised or sedentary mice into the left cerebral ventricle.