DNA Sequencing of Parathermosynechococcus lividus PCC 6715 cultivated in a Floating Light Ball Reactor (FLBR)

Next Generation Sequencing was conduct by STAR*SEQ GmbH laboratory (Johann-Joachim-Becher-Weg 30a, 55128 Mainz). Harvesting cells from the cyanobacteria strain PCC6715, preparation of genomic DNA using the Maxwell® RSC Fecal Microbiome DNA Kit, Qiagen QIAamp PowerFecal DNA Kit or Quick-DNA Fecal/Soil Microbe Kit. Grinding of samples will be done using Bead Tubes (glass beads). Preparation of a DNA-seq library and paired-end sequencing, 10 M reads, read length 150 nt, NextSeq 2000, XLeap SBS chemistry, the estimated output is up to 10 M reads in total (2x5 M reads) and up to 1.5 Gb per sample. Sequencing library preparation with NEBNext® UltraExpress™ FS DNA Library Prep Kit. Kraken2 Metagenomic Analysis: The Kraken Metagenomics workflow assigns taxonomic labels to short DNA sequences with high sensitivity and speed using exact alignments of k-mers and a novel classification algorithm. The DNA of strain PCC6715, as well as contaminants of both eukaryotic and prokaryotic origin, were sequenced and analyzed. Sequencing individual DNA fragments allows for both qualitative and quantitative analysis of the contaminants.

Sample FL-013-020 obtained from isolated wet biomass of non-sterile cultivaton of Parathermosynechococcus lividus PCC6715 at 55 °C in 1-Liter glass bottles in BG-11 medium.
Sample FL-010-221 obtained from isolated wet biomass of sterile cultivaton of Parathermosynechococcus lividus PCC6715 at 40 °C in 1-Liter glass bottles in BG-11 medium.
Sample FL-009-063 and Sample FL-009-064 are duplicates obtained from isolated wet biomass of non-sterile cultivaton of Parathermosynechococcus lividus PCC6715 at 45 °C in BG-11 medium in a Floating Light Ball Reactor (FLBR) at 22-Liter scale.