Transcriptome profile of Haloferax volcanii H26 strain under sodium bisulfite induction (RNA-seq)

DNA deamination occurs constantly in a cell and causes DNA damage. As this damage can be deleterious, organisms have evolved many systems to eliminate it. Deamination of cytosine, guanine, adenine, and 5-methylcytosine results in the formation of uracil, xanthine, hypoxanthine, and thymine, respectively. Sodium bisulfite is a kind of DNA deaminating agent that can increase the frequency of DNA deamination in cells. This study measures the transcriptome profile of Haloferax volcanii H26 strain and HVO_RS06830 gene knockout strain, induced with different concentrations of sodium bisulfite. Haloferax volcanii H26 strain and HVO_RS06830 gene knockout strain were grown in Hv-YPC medium at 45°C until OD600 reach 0.5. Subsequently, sodium bisulfite was added to the final concentration of 0.1 or 0.5 mM and continuously cultured for 48 h. Cells were harvested by centrifugation at 12,000 rpm at 4°C for 20 min. After that, total RNA was extracted immediately using Direct-zol RNA MiniPrep Kits. Sequencing libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s recommendations, and sequenced by Illumina NovaSeq6000 PE150 platform. The experiment was repeated two times.