Pig pluripotent stem cell and stem cell-derived macrophage RNA sequencing

Pig pluripotent stem cell (PSCs) lines are an alternative source of phenotypically normal macrophages. The stem cell lines are derived either directly from embryos or generated through induction of pluripotency through factor-directed reprogramming. PSCs can be expanded indefinitely in culture, are amenable to most gene manipulation techniques, and can be differentiated into a variety of cell types in culture including macrophages. Based on their gene expression, the in vitro-derived macrophages closely resemble myeloid cells normally produced in the embryonic yolk sac that then colonise the foetus and contribute to the tissue resident macrophages of the adult. Despite their in vitro origins, PSC-derived macrophages exhibit similar phenotypic plasticity to ex vivo cells, and will adopt features of mature tissue resident characteristics in response to environmental cues.Please contact Tom Burdon ([e-mail]) or Alan Archibald ([e-mail]) if you have questions about the data.