CRISPR screens identify gene targets at breast cancer risk loci [ATAC-seq]

Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely modulate cancer risk by regulating gene expression. However, pinpointing the exact target of the association, and identifying the phenotype it mediates, is a major challenge in the interpretation and translation of GWAS. Transcriptome, open chromatin, and chromatin interaction profiling using RNA-seq, ATAC-seq and H3K27ac-mediated HiChIP of six immortalized mammary epithelial cell lines representing breast cells with either a luminal progenitor signature (K5+/K19+, K5+/K19-), a mesenchymal signature (B80-T17, mesHMLE) or a more epithelial like signature (B80-T5, HMLE)