Purinergic Preconditioning Induces Epigenomic and Transcriptomic Changes Resembling Epilepsy-associated Microglial States . Purinergic Preconditioning Induces Epigenomic and Transcriptomic Changes Resembling Epilepsy-associated Microglial States

DNA methylation profiling of monocytes and monocyte-derived microglia like cells. Innate immune memory was explored in microglia-like cells through consecutive exposure to ATP and LPS.The Illumina Infinium MethylationEPIC Beadchip was used to obtain DNA methylation profiles across approximately 850,000 CpGs. Overall design: Bisulphite converted DNA from the 20 samples were hybridised to the Illumina Infinium MethylationEPIC Beadchip.