Quantitative assessment of porin-mediated solute transport in biomimetic membranes

This repository contains the raw data of the experiments related to the paper entitled: "Quantitative assessment of porin-mediated solute transport in biomimetic membranes". The content entails data that is not included in the main manuscript or in the supplementary data. 

Short description of the paper:

The paper investigates substrate transport through bacterial porins reconstituted in synthetic polymersomes. Transport was monitored in real time by encapsulating Gaussia luciferase, which reports on coelenterazine (CLZ) translocation across the membrane. The dataset enables quantitative analysis of porin-mediated permeability and transport kinetics. Three porins were compared: the wild-type OmpF, the variant OmpF∆ of larger pore size, and PhoE. Measurements include transport rates for CLZ as well as the two derivatives CLZ-I and CLZ-n with higher molecular mass and hydrophobicity, allowing insights into substrate selectivity.

About the dataset:

The dataset contains the raw data of the four polymersomes configurations PolGLControl, PolGLOmpF, PolLOmpF∆, and PolGLPhoE used in the experiments of the paper:

Polymersome analysis:

DLS measurements

Zeta View: Report

Mass transport

Luminescence assay data

Calibration data

Researchers are welcome to reanalyze or model the translocation kinetics using their own approaches. 

Further References:

This work was supported by DFG Project 290825040,  and the Federal Ministry of Research, Technology and Space (BMBF) – founding number 6KIS1987.

This work is also funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – GRK 2950 – Project-ID 509922606:  Synthetic Molecular Communication Across Different Scales: From Theory to Experiments (SyMoCADS).

For more information visit: Institute of Digital Communication, Institute of Bioprocess Engineering.