Microarray analysis of palatal shelves from wild-type versus p63-null mouse embryos

Mutations in the transcription factor p63 underlie of a series of human malformation syndromes which are defined by a combination of epidermal, limb and craniofacial abnormalities including cleft lip and palate. Transcription profiling was performed to determine the role of p63 in vivo mouse palatal shelves. Microarray analysis was done of palatal shelves dissected from E14.0 wild-type versus p63-null mouse embryos.转录因子p63（transcription factor p63）的突变是一系列人类畸形综合征的致病基础，此类综合征以表皮、肢体与颅面部异常为特征，其中包括唇腭裂。为探究转录因子p63在小鼠腭突中的体内生物学功能，本研究开展了转录谱分析（Transcription profiling）。具体而言，研究者对取自胚胎发育第14.0天（E14.0）的野生型（wild-type）与p63基因缺失型（p63-null）小鼠胚胎的腭突组织，实施了微阵列（Microarray）分析。