Epistasis analysis of <i>elt-1(ku491); daf-12(rh61rh411)</i> mutant phenotypes with RNAi of other heterochronic genes.

aL4 Bursting Vulva, Alae Formation, and Young Adult Seam cell phenotypes were analyzed for strains of indicated genotypes and RNAi treatment. elt-1(rf) is the partial loss-of-function allele ku491. daf-12(lf) is the loss-of-function allele rh61rh411. bFor bursting vulva rate, the p-value for the comparison of elt-1(ku491); daf-12(lf) animals on empty-vector (-) RNAi vs target gene RNAi is < 0.0001 for each of lin-28, hbl-1, lin-14, lin-41 and mab-10, 0.003 for lin-46, 0.0002 for lin-42, 0.983 for kin-20, 0.0003 for ceh-16, and 0.315 for dre-1. cFor seam cell numbers, the p-value for the comparison of elt-1(ku491);daf-12(lf) animals on empty-vector (-) RNAi vs on target gene RNAi is <0.0001 for lin-28, hbl-1, lin-14, lin-41, lin-46, lin-42, kin-20, mab-10, ceh-16, and dre-1, and > 0.9999 for lin-29. Abbreviations: e.v., empty vector; Abs, absent; Gap, gapped; Pres, present; #, seam cell number measured using the scm::GFP reporter; Std Dev, standard deviation. Epistasis analysis of elt-1(ku491); daf-12(rh61rh411) mutant phenotypes with RNAi of other heterochronic genes.