Comparison of isogenic lines differing in vernalization requirement

Gene expression was assyed in seedlings of isogenic barley lines differing in vernalization requirement. Seedlings were harvested after 5 days germination in darkness at 20 degrees. Seedlings were then subjected to 1 day at 4 degrees after 5 days germination in darkness at 20. Genotypes include the winter barley parent Dairokakku, and isogenic lines carrying a deletion of VRN2, active VRN1 allleles (VRN1-10, or VRN1-8) or an active allele of FT1/VRN3. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Trevaskis. The equivalent experiment is BB97 at PLEXdb.] genetic line: +VRN1-10 - temperature: 20 C(3-replications); genetic line: +VRN1-10 - temperature: 4 C(3-replications); genetic line: -VRN2 - temperature: 20 C(3-replications); genetic line: -VRN2 - temperature: 4 C(3-replications); genetic line: +VRN1-8 - temperature: 20 C(3-replications); genetic line: +VRN1-8 - temperature: 4 C(3-replications); genetic line: +FT1/VRN3 - temperature: 20 C(3-replications); genetic line: +FT1/VRN3 - temperature: 4 C(3-replications); genetic line: Parent (winter type) - temperature: 20 C(3-replications); genetic line: Parent (winter type) - temperature: 4 C(3-replications)