RNAseq of whole striatum from hypothyroid mice, and hypothyroid mice treated with thyroid hormone for 2 days.

Striatum in rich in GABAergic neurons which require thyroid hormone for their maturation. We used propyl thio uracyl to make mice hypothyroid, and then restored the hormonal level using thyroid hormone. This provided 3 groups of mice for striatum RNAseq analysis. Our results point to GABAergic neurons as direct targets of thyroid hormone during brain development and suggest that many defects seen in hypothyroid brains may be secondary to GABAergic neuron malfunction. Overall design: All mice treated with PTU and then some treated with T3