A FLIM Microscopy Based on Acceptor-Detected Förster Resonance Energy Transfer
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https://figshare.com/articles/dataset/A_FLIM_Microscopy_Based_on_Acceptor-Detected_Fo_rster_Resonance_Energy_Transfer/14195808
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资源简介：
Time-resolved donor-detected
Förster resonance energy transfer
(trDDFRET) allows the observation of molecular interactions of dye-labeled
biomolecules in the ∼10–100 Å region. However,
we can observe longer-range interactions when using time-resolved
acceptor-detected FRET (trADFRET), since the signal/noise ratio can
be improved when observing the acceptor emission. Therefore, we propose
a new methodology based on trADFRET to construct a new fluorescence
lifetime microscopy (FLIM-trADFRET) technique to observe biological
machinery in the range of 100–300 Å in vivo, the last
frontier in biomolecular medicine. The integrated trADFRET signal
is extracted in such a way that noise is canceled, and more photons
are collected, even though trADFRET and trDDFRET have the same rate
of transfer. To assess our new methodology, proof of concept was demonstrated
with a set of well-defined DNA scaffolds.
创建时间：
2021-03-23
