Thermal stress in lobsters affects cardiac lipidome

Lipids are fundamental components of many biological structures, and their composition is diet dependent. Differences in lipid composition can impact the functioning of cellular membranes and proteins, subsequently altering the organism’s ability to respond to environmental conditions. The American lobster (Homarus americanus) is an economically important shellfish in New England and is frequently kept in lobster impoundments (pounds) for prolonged periods where they are typically fed herring, which differs from the natural diet of wild-caught lobsters. In this study, we compared the lipid composition of lobster muscle, heart, and hepatopancreas from wild caught and from pound kept lobsters that were fed different diets. We performed lipidomic analysis, mitochondrial function tests, tested gene expression of cellular stress markers, and evaluated thermal stress tolerance assessing heart and ventilation rate, as well as hemolymph oxygenation during a fast progressive temperature challeng..., Frozen tissue samples were used in mass spectrometry-based multiple precursor ion scan (MPIS) lipidomic analysis (Simons et al., 2012) at the MaineHealth Institute for Research (MHIR) Proteomics and Lipidomics core facility in Scarborough, Maine. Lipids were isolated using a modified Bligh and Dyer protocol (Bligh and Dyer, 1959; Liaw et al, 2016; Okeoma et al., 2024) using dichloromethane and methanol. Briefly, around 20 mg of tissue was transferred to an Eppendorf cup with 200 µL of 66% methanol (MeOH) (Alfa Aesar-Themo Fisher, Waltham, MA, USA) in LC-MS grade water (Burdick and Jackson-Honeywell, Muskegon, MI, USA) and kept on ice all the time. Tissues were homogenized with a pellet pestle motor (Kimble, Millville, NJ, USA) and then by sonication using a Branson Sonifier 250 (VWR Scientific, Radnor, PA, USA) for ten 30-second cycles at 30% until the tissue was dissolved. Samples sat on ice for 10 min to allow for debris to settle. Supernatants were then transferred to a glass screw-c..., , # Lobster diet affects lipidomics and thermal tolerance

[https://doi.org/10.5061/dryad.9w0vt4brt](https://doi.org/10.5061/dryad.9w0vt4brt)

## Description of the data and file structure

Lobsters were caught off the Maine coast (wild caught) or purchased from a local lobster pound (Pound) and then kept in a flow through aquarium for about 150 days and fed typical pound diet (herring) or wild diet (mussel) leading to 4 groups: pound kept pound-fed lobsters (PKPF) wild-caught, pound-fed lobsters (WCPF) pound-kept, wild-fed (PKWF) and wild-caught, wild-fed (WCWF).

Heart tissue was extracted after 150 days of differential feeding herring or mussels and analyzed by mass spectrometry-based multiple precursor ion scan (MPIS) for lipid composition.

### Files and variables

#### File: Lobster_lipidome-Positive.xlsx

**Description:** This dataset represents **lipidomics data** — specifically, measurements of **sphingomyelin (SM) species** detected in a series of biological or experimental sa...,