Genotypes, allele frequencies, and geographic locations of the Native American populations investigated.

1Samples genotyped in present study = 229;2Caution is needed regarding the classification of these modes of subsistence, since they are not stable over time and may not be unique. However, the two categories adopted here (agriculturalist and hunter-gatherer/forager) represent general pre-Columbian subsistence conditions of the investigated populations in accordance with what is known about them. AMOVA results: (a) Among the subdivisions (FCT): 3.6% (p = 0.000); (b) Among populations within the Mesoamerican Agriculturalist subdivision (FST): 1.8% (p = 0.008); (c) Among populations within the South American hunter-gatherer/forager subdivision: 5.3% (p = 0.005); Among populations within the Andean Agriculturarist group: 0% (p = 0.36).