The transcriptome of WT and DDX55 knockout CD4 naive T cells that activated for indicated time points

WT and DDX55 knockout CD4 naive T cells were stimulated with anti-CD3/CD28 antibodies in vitro for 0, 8, 24 hours, and the transcriptome of these cells were determined by whole-transcriptome sequencing. Overall design: Purified WT or Ddx55-KO naïve CD4+ T cells were stimulated with plate-bound anti-CD3(5 ug/ml) and anti-CD28(2.5 ug/ml) for 0, 8, and 24 hours followed by total RNA isolation. RNA library preparation and high throughput sequencing were conducted