Variation and positive selection measures of sites at the mammalian ACE2/SARS-CoV-2 binding interface.

The 20 sites in the ACE2 receptor that are involved in binding the SARS-CoV-2 spike protein are shown. For each site, measures of normalized evolutionary rate by Rate4Site [20], amino acid variation using Shannon entropy [21], branch-scaled dN-dS from SLAC [22], and likelihood ratio test (LRT) from MEME [23] are included. The measures are shown when computed using all mammalian sequences, as well as when using sequences from each of the six species groups (all considered mammalian species, all mammalian species excluding known hosts of SARS-like viruses, mammalian species excluding primates and rodents, mammalian species excluding primates, rodents, and known hosts of SARS-like viruses, primates only, and rodents only). (XLSX)