Transcriptome profiling of in-vitro challenged monocyte-derived macrophages during pre- and post infection stages in Indian Ghurrah and Landrance pigs

In this study, the monocyte-derived macrophages (MDMs) were cultured and challenged with CSF virulent virus strain and response was studied at 48 hours post-infection. Transcriptome profiles were generated in the MDMs of 3 Landrace, and 3 indigenous Ghurrah pigs before and 48 hours after challenge with Classical Swine fever virulent virus strain under in-vitro conditions. The piglets were 3 months old and free of maternally derived antibodies against CSF. The sequencing was done on Illumina X-Ten platform, generating average of 69.78 million paired end reads for each sample. Overall design: Horizontal and vertical comparisons of MDM transcriptomes were made along challenge (pre- versus post challenge) and breed (Ghurrah versus Landrace).