PXLP-K341-GPT2 transaminates PYR to form L-Ala

Glutamic-pyruvate transaminase 2 (alanine aminotransferase 2) (GPT2) catalyzes the reversible reaction of pyruvate and glutamate to form alanine and 2-oxoglutarate (alpha-ketoglutarate) (Yang et al. 2002). Unpublished crystallographic data are consistent with a homodimeric structure for the enzyme with one molecule of pyridoxal phosphate associated with each monomer (PDB 3IHJ). Recent studies of organelles purified from cultured human muscle cells suggest that GPT2 is localized to mitochondria (Glinghammar et al. 2009).

谷氨酸-丙酮酸转氨酶2（丙氨酸转氨酶2，GPT2）催化丙酮酸与谷氨酸的可逆反应，生成丙氨酸和2-酮戊二酸（α-酮戊二酸）（杨等，2002年）。未发表的晶体学数据与酶的均聚二聚体结构一致，每个单体与一个吡哆醛磷酸分子相关联（PDB 3IHJ）。近期研究表明，从培养的人肌细胞中纯化的细胞器表明GPT2定位于线粒体中（Glinghammar等，2009年）。