Functional characteristics of the alanine insertion mutant currents.

*(p<0.05) and
**(p<0.0002) indicates significant difference from WT by one way ANOVA using Dunnett's multiple comparison post hoc test. None of the Hill coefficients were significantly different than WT.
#The solution exchange rate was measured by the change in membrane current of a voltage clamped oocyte in response to a switch from Na+ to K+ containing buffer solution (see Methods section for details). The current change was best fit by a double exponential function. The percent of each component is given.