Data underlying the publication: Bacterial homologs of innate eukaryotic antiviral defenses provide phage protection.

This dataset accompanies the study <em>“Bacterial homologs of innate eukaryotic antiviral defenses provide phage protection”</em> and contains experimental data generated to investigate how bacterial defense systems share functional similarities with eukaryotic antiviral mechanisms. The research objective was to identify, characterize, and functionally validate bacterial genes that act as homologs of innate immune components, with a focus on their ability to protect against bacteriophage infection. Data were collected through a combination of comparative genomics, molecular cloning, genetic knockouts, phage infection assays, and high-throughput sequencing to assess bacterial survival and phage replication. The dataset includes genomic annotations, experimental measurements of phage resistance, phylogenetic trees, HMM profiles, pangenomic analyses, alphafold results, all supplementary data from the publication and sequencing-based readouts, providing both raw and processed data suitable for further validation, comparative analysis, and reproduction of the study’s findings.

本数据集配套于研究论文《真核生物先天抗病毒防御系统的细菌同源物可提供噬菌体保护》（*Bacterial homologs of innate eukaryotic antiviral defenses provide phage protection*），包含为探究细菌防御系统与真核抗病毒机制的功能相似性而生成的全部实验数据。本研究的核心目标为鉴定、表征并功能验证作为真核先天免疫组分同源物的细菌基因，重点探究其抵御噬菌体感染的能力。研究数据通过比较基因组学、分子克隆、基因敲除、噬菌体感染实验以及高通量测序等多种技术手段联合采集，用于评估细菌存活率与噬菌体复制水平。本数据集涵盖基因组注释、噬菌体抗性实验测量结果、系统发育树、隐马尔可夫模型（Hidden Markov Model, HMM）谱、泛基因组分析（pangenomic analysis）结果、AlphaFold预测结果、论文全部补充数据以及测序读取数据，包含原始数据与处理后数据，可用于进一步验证、比较分析以及复现该研究的实验结论。