LC-MS/MS Analysis.

Data are presented in 9 different tabs. The “Results Summary” tab provides an overview and heat map of the top protein hits identified for each of the three different conditions (Tz-HFF: tachyzoite fibroblast infection; Bz-HFF: bradyzoite fibroblast infection; Neuron: neuron infection). The tabs labeled “Condition_PeptideCalc” demonstrate the analysis pipeline, including equations used in Excel to ultimately calculate log2 protein fold changes and p-values. The tabs labeled “Condition_VolcanoPlot” provide a graphical overview of protein fold changes and short names for the top protein hits. Lastly, the “Peptide_RawData” tabs provide identification scores and PEP values for each unique peptide identified from either Toxoplasma-Human or Toxoplasma-Mouse combined proteome database searches.
(XLSX)