Next Generation Sequencing Quantitative Analysis of altered expression of genes in circNCOR1 overexpressing bladder cancer cells

Bladder cancer (BCa) is one of the most common genitourinary malignancies worldwide, with approximately 429,800 new cases and 165,100 deaths annually in the world. Recently, circular RNA (circRNA), a class of non-coding RNA generated from pre-mRNA back splicing, has emerged as crucial mediator in various tumor initiation and progression. To identify molecular mechanism of circNCOR1 in the progression of BCa, Next generation sequencing (NSG) was performed to explore the target genes of circNCOR1 in BCa. Overall design: To detect the precise mechanism of circNCOR1 in LN metastasis of BCa, the total RNA of UM-UC-3 cells transfected with Vector plasmids or circNCOR1-overexpressing plasmids was extracted and the next generation sequencing was conducted to explore the expressing profile difference between Vector and overexpression group. The result was obtained from three independent experiments.