Hepatic gene expression profiling in AHNAK KO mice

To identify molecular mechanism underlying the protection from diet-induced hepatic steatosis in AHNAK deficiency mice, we examined microarray analysis with liver sample from HFD-fed AHNAK KO and WT mice. Two-condition experiment, regular chow (CD) -fed WT vs. CD-fed AHNAK KO and High fat diet(HFD)-fed WT vs. HFD-fed AHNAK KO mice. Biological replicates: 3 control, One replicate per array.为解析AHNAK缺陷小鼠抵御饮食诱导性肝脂肪变性的分子机制，本研究针对高脂饮食（High Fat Diet, HFD）喂养的AHNAK基因敲除（Knockout, KO）与野生型（Wild Type, WT）小鼠的肝脏样本开展了微阵列分析（microarray analysis）。本实验为双条件对照实验，包含两组对比：其一为常规饲料（regular chow, CD）喂养的野生型小鼠与常规饲料喂养的AHNAK敲除小鼠，其二为高脂饮食喂养的野生型小鼠与高脂饮食喂养的AHNAK敲除小鼠。生物学重复设置为3例对照样本，每个微阵列芯片对应一次生物学重复。