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NGS data of CRISPR-induced indels

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA977205
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We provide NGS data of different CRISPR-induced indels. The preprint paper "A PCR-RFLP method for the detection of CRISPR-indused indels" describes a new method (PIM-RFLP) that we developed for the detection of CRISPR-induced indels. PIM-RFLP was applied to CRISPR-edited Neuro-2a cells in two different loci (Abracl and Hprt). The efficiency of the method was validated through NGS (Ion Torrent) of the PCR products encompassing the Double Strand Break.
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2023-05-29
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