Table 8 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

Table 8 Varying single nucleotide positions among ITS sequence variants within Barbarea vulgaris including newly obtained and publicly available sequences by mid-2019 (Fig. 8). In total seven groups of sequence types were found, among which group 2 and 3 differ from the G-type only by additional sequence ambiguities not indicated here. Eight sites are distinguishing between P- and G-types as indicated in bold.
Sequence group No. aNumber of accessionsType or subspeciesPosition in alignment b8183181251452474565586617631P-type cCAGCCATTC121G-type cTTATCGCCT79“ rivularis ” cTTATTGCTT43(PxG?)dCTAY eCGTTT52–CTGTTGCTT21–TTATCGCCT31–TTATCGCCT
a See Supplementary Table S1 and Fig. 8. GenBank accession codes for the newly acquired sequences are: Group 6 (P-type), MN508440; Group 1 (G-type and ssp. vulgaris), MN508439.
b Refer to the alignment in Supplementary Table S2.
c The designations of P- and G-type represents the majority of G-type and P-type accessions in Supplementary Table S1. Sequences from material identified as ssp. rivularis or ssp. vulgaris tended to cluster in sequence group 7, although a few ssp. vulgaris sequences were also found in sequence group 1.
d Preliminary interpretation of the intermediacy of the sequence: DNA sequence information and phylogenetic position in the network of accessions from group 4 might indicate that these accessions represent hybrids between the two clearly separated clades found within B. vulgaris according to the SplitTree analysis.
e Y: sequence ambiguity, indicating multiple ITS copies within a single individual with differing nucleotides (C or T).