Assessing Hermetia illucens solinvivirus infection in BSF adults and anti-viral reponses

To assess the replication levels of Hermetia illucens solinvivirus, Hermetia illucens insevirus and Hermitia illucens sigmavirus after orally infecting BSF adults with partially purified Hermetia illucens solinvivirus. The infection levels were observed over 9 days using qPCR. For timepoint days 3, 5 and 7, total RNA and small RNA NGS datasets were obtained after pooling two individuals per time point during an infection experiment. Both males and females were collected separately. Adults from two different BSF rearing colonies, each reared under slightly different conditions to represent two separate rearing facilities.