Gene expression profiling in Bmi1 knockout innate B-1a cells

In order to identify the key genes for self-renwal abiity of innate immune B-1a cells, we have gene expression analysis using Aglinant Whole Mouse gGenome Oligo Microarrays performed by Miltenyi Biottec. We isolated peritoneal B-1a cells from wild type mice and Bmi1 kockout (KO) mice that lost self-renewal ability of B-1a cells, and submitted purified RNA from each samples to Miltenyi. The peritoneal B-1a cells were isolated from wild type mice and Bmi1 KO mice. Total 7 samples from Bmi1KO and 7 samples from WT mice from 2 sets of expeiments were submitted to the Whole genome micro array analysis.