Text S1 - When the Most Potent Combination of Antibiotics Selects for the Greatest Bacterial Load: The Smile-Frown Transition

Supporting information. This file includes nine sections and two appendices: 1. Introduction: hit early, hit hard? 2. Drug interaction profiles: synergy and antagonism. 3. Experimental evolution in a two-drug environment: methods. 4. Experimental evolution in a two-drug environment. 5. Analysis: whole genome sequencing. 6. Analysis: a mathematical model consistent with data. 7. Validating the theory: testing the smile-frown experiment with an acrAB knockout. 8. Optimal drug combinations are not constant: an analysis. 9. Final comment: single cell synergy and population synergy. Appendix A: Parameter values. Appendix B: Genes annotated in the duplicated regions. (PDF)