Genome-wide mapping of MEF2A binding sites by CUT&Tag-seq in chicken primary myoblasts

In this study, CUT&Tag-seq technology was employed to investigate MEF2A binding sites across the entire genome of chicken primary myoblasts. CUT&Tag was performed using CUT&Tag Assay Kit for Illumina Pro (TD904-1) from Vazyme. The negative control group utilized Rabbit IgG (GB111738, Servicebio Co., Ltd., Wuhan, China), while the trial group employed the Anti-MEF2A Rabbit pAb (GB11965, Servicebio Co., Ltd., Wuhan, China).The final DNA library on a HiSeq PE150 platform was subjected for the analyses. This study provides a wide landscape of MEF2A target genes from chicken primary myoblasts, which supports the active role of MEF2A in avian muscle development.