Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators.

This upload contains all data collected and presented in (https://doi.org/10.1101/2023.04.13.536801). The files are zipped files associated with main figures, supplemental figures, and supplementary tables. We have additionally attached a .zip file that includes the Chen1 and Dana2 datasets used to run our model, as well as an amino acid property matrix derived from AAINDEX3. Dataset Refs: 1. Chen, T.-W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295–300 (2013). 2. Dana, H. et al. High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat. Methods 16, 649–657 (2019). 3. AAindex. (https://www.genome.jp/aaindex/)