淫羊藿药材化学成分检测数据集

1、确定检测目的和检测对象：针对淫羊藿药材，构建“基于特征标志物的HPLC定量测定”“基于多成分的HPLC含量测定”“基于UPLC-QTOF-MS结合代谢组学技术的柔毛淫羊藿和箭叶药材中特征标志物测定”“基于 UPLC-QTRAP-MS/MS 法测定12个成分的定量分析方法”“基于22个指标成分的多角度分析”的分析内容。
2、确定检测方法：根据分析内容的目标，明确测定方法和方法学验证内容，包括对照品及供试品的制备方法、测定设备、测定条件、考察因素、指标成分等内容。
3、获取检测结果：收集高效液相色谱图、GraphPad小提琴图、Origin热图等；
4、生成检测数据集：利用常规数据分析方法，如化学计量法、多元变量统计分析、热图分析、初步表观分析等对检测数据进行分析，获得不同基原的淫羊藿的化学组分和含量数据、不同种的箭叶淫羊藿的化学成分组成和含量数据、柔毛淫羊藿与箭叶淫羊藿中化学成分的差异数据、柔毛淫羊藿与箭叶1号淫羊藿采收产质量数据、柔毛淫羊藿与箭叶1号淫羊藿化学成分含量与采收月份关联数据。

1. Define detection objectives and test objects: Aiming at Epimedium medicinal materials, construct the following analytical contents: HPLC quantitative determination based on characteristic markers; HPLC content determination based on multiple components; Determination of characteristic markers in Epimedium pubescens and Epimedium sagittatum using UPLC-QTOF-MS combined with metabolomics technology; Quantitative analytical method for determination of 12 components via UPLC-QTRAP-MS/MS; Multi-angle analysis based on 22 indicator components.
2. Specify detection methods: Clarify the determination methods and methodological validation contents based on the objectives of the analytical contents, including preparation methods of reference substances and test samples, detection equipment, determination conditions, investigation factors, indicator components, and other relevant details.
3. Acquire detection results: Collect high-performance liquid chromatography (HPLC) chromatograms, GraphPad violin plots, Origin heatmaps, and other related experimental charts.
4. Generate detection datasets: Analyze the detection data using conventional data analysis methods such as chemometrics, multivariate statistical analysis, heatmap analysis, and preliminary apparent analysis, to obtain: chemical component compositions and content data of Epimedium with different germplasm origins; chemical component compositions and content data of different Epimedium sagittatum accessions; chemical component difference data between Epimedium pubescens and Epimedium sagittatum; harvest yield and quality data of Epimedium pubescens and Epimedium sagittatum No.1; correlation data between chemical component contents and harvesting months for Epimedium pubescens and Epimedium sagittatum No.1.

搜集汇总

背景与挑战

背景概述

该数据集专注于淫羊藿药材的化学成分检测，采用高效液相色谱、质谱等多种分析技术，构建了多角度的定量测定方法，包括特征标志物识别和多成分含量分析。通过方法学验证和数据分析，提供了不同基原和种类淫羊藿的化学组分、含量差异数据，并关联了采收月份与化学成分变化，支持药材质量评估和研究。