Chromosome-level assembly of two pearl millet (Cenchrus americanus) genomes, functional annotation and transcriptomes

We present platinum-grade reference genome assemblies, featuring gapless chromosomes, for two pearl millet lines: the Striga-susceptible SOSAT-C88-P10 (P10) and the resistant 29Aw (Aw). This study was motivated by the severe impact of the root parasitic weed Striga hermontica on pearl millet yield, with Striga relying on host-released strigolactones (SLs) for seed germination. These resources enable advanced genomic investigations in pearl millet, facilitating research on phenotypic traits, diversity, and adaptation. Additionally, comparative analysis between P10 and 29Aw genomes can pave the way for identifying genetic determinants of Striga resistance, contributing to the development of resilient pearl millet lines.

Methods
For a detailed workflow of the genome annotation part of the work, please refer to the following github repo: https://github.com/mjfi2sb3/millet-genome-annotation