Expression data of synchronised primary human fibroblasts (NHDF, Data Set 2)

Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other. We used gene expression microarrays to analyse the global programme of gene expression of human primary fibroblasts from a quiescence state (G0) to a synchronised cell proliferation Primary human fibroblasts (NHDF) were firstly synchronised by limiting nutrients (from 10% to 0.1% of faetal calf serum) in the medium for 48 h. Samples were collected at 0 h (after 48 h of starvation but before full medium replacement), 1 h and every 2 h thereafter up to 24 h. Two biological replicas for controls were collected (unsynchronised).