RNA seq of cardiomyocytes (CMCs) and vascular smooth muscle cells (SMCs) of CMS/SMC-specific TEAD1-KO and control mice

SM22a-Cre+/TEAD1F/W male mice were bred with TEAD1F/F/mTmG+/+ female mice to generate cardiomyocyte (CMC) / vascular smooth muscle cell (VSMC)-specific TEAD1 deletion with GFP tag. GFP positive heterozygous control and TEAD1 KO cardiac and DA tissues were dissected under a fluorescent stereoscope. Subsequently the GFP positive CMCs and VSMCs were dissociated by enzymatic digestion and collected by fluorescence activated cell sorting (FACS). Total RNA was then extracted from the purified GFP positive cells using RNeasy Micro Kit (Qiagen) and subjected to whole transcriptome RNA-seq analysis. Differential gene expression analysis was performed at the Genome Technology Access Center at Washington University. Following amplification of the input RNA by Sigma SeqPlex RNA amplification kit, library was prepared and single-end sequencing in 50-bp length was performed on a HiSeq 3000 system (Illumina).