CO1 and ITS alignments of bladder grasshopper species

Polymorphism in one sex often occurs amongst arthropods, and is often linked with alternate mating strategies. Bladder grasshoppers show distinct polymorphism resulting in two different male morphs utilizing two different mating strategies. Primary males are larger in size and make use of acoustic communication to attract mates, while secondary (alternate) males are significantly smaller in size, flightless, and do not call, making use of a “sneaker” or satellite strategy. Three species of bladder grasshoppers have been described (Parabullacris vansoni, Paraphysemacris spinosus, and Pneumoracris browni) that only have an alternate male morph and no primary male, whereas all other species in the family are described as having only a primary male. However, it is now known that both types of males occur in at least three species (Bullacris discolor, B. membracioides, and B. obliqua). Thus, the validity of the taxonomic descriptions for species with no identified primary male has come into ..., Specimens used for this study were from previous collections that had been preserved in 90–100% ethanol. Collection dates span many years. 
DNA extraction
PCR and gel electrophoresis
Sequences alignments and phylogenetic analysis, , # CO1 and ITS alignments of bladder grasshopper species

[https://doi.org/10.5061/dryad.0rxwdbsbp](https://doi.org/10.5061/dryad.0rxwdbsbp)

## Description of the data and file structure

Bladder grasshoppers collected over decades, preserved in ethanol, were used in this genetic study. 

### Files and variables

#### File: CO1_alignment.fas

**Description:** 71 sequences successfully extracted for phylogenetic analysis of the COI gene marker. This fasta file is the alignment of those sequences. 

#### File: ITS_alignment.fas

**Description:** 63 sequences successfully extracted for phylogenetic analysis of the ITS gene marker. This fasta file is the alignments of those sequences.

## Code/software

BioEdit

,