The increase of miR-378a-3p in keratinocyte associated with pathogenesis of psoriasis

We report the high-throughput profiling of human primary keratinocytes transfected with either miR-378 mimic or miR-378 inhibitor at 24 or 48 hours. Our study focused on the dysregulation of microRNAs that might partially be a fundamental mechanism leading to psoriasis development. We knock-in and knock-out a specific miR-378 and see the cellular responses. As expected, overexpression of miR-378a inhibited the proliferation, enhanced the apoptosis and disturbed the cell cycle. Regarding to the confirm experiment, our results suggested that the expression of miR-378a associated with the psoriasis pathogenesis enhancing the severity of psoriatic lesion, and might useful for further development of therapeutic strategy for psoriasis. We investigated microRNA expression in psoriatic skin. Experiment found miR-378 was dsyregulated in the skin biopsy from patients. To investigate the entire effects of miR-378, overall design was eventually come out with RNA-sequencing on 4 groups at 2 timepoints (24 and 48 hours). These are including miR-378 mimic transfection, control mimic transfection, miR-378 inhibitor transfection, and control inhibitor transfection.