Yagi et al. Figure data

The files contain the data underlying the figures in: Moeri Yagi, Simon Miller, Yoshiko Nagai, Shinsuke Inuki, Ayato Sato, and Tsuyoshi Hirota, A methylbenzimidazole derivative regulates mammalian circadian rhythms by targeting Cryptochrome proteins. F1000Research

Figure 1B.csv (Luminescence rhythms of Bmal1-dLuc and Per2-dLuc U2OS cells in the presence of various concentrations of TH401 (n = 2))
Figure 1C.csv (Changes in period (n = 6))
Figure 1D.csv (Changes in luminescence intensity (n = 6))
Figure 1E.csv (Changes in cellular ATP levels after treatment with various concentrations of TH401 (n = 4))
Figure 2A.csv (Inhibitory effect of Longdaysin and TH401 on CKIδ activity in vitro (n = 1))
Figure 2B.csv (Changes in the half-lives of CRY-luciferase fusion proteins (CRY1-LUC and CRY2-LUC) relative to LUC in the presence of various concentrations of TH401 (n = 2))
Figure 2C.csv (Changes in Per2::Luc knock-in reporter activity in wild type, Cry1/Cry2 double knockout, Cry1 knockout, and Cry2 knockout fibroblasts (n = 4–8))
Figure 2D.csv (Changes in the cellular circadian period of Bmal1-Eluc reporter rhythms in Cry1/Cry2 double knockout fibroblasts rescued with CRY1 and CRY2 (n = 3–6))
Figure 2E.csv (Changes in the protection of CRY1 and CRY2 proteins from thermal denaturation in HEK293T cells (n = 4))
Figure 2F.csv (Changes in denaturing temperatures of recombinant CRY1(PHR) and CRY2(PHR) in vitro (n = 2))
Figure 4.csv (Changes in the circadian period in primary screening (tested at 7 μM; n = 1) and a secondary assay (tested at 24 or 8 μM; n = 3))