Analysis of gene-expression in normal, unmanipulated naive (CD44lo/CD49dlo; CD5lo/CD5hi) and Virtual memory (CD44hi/CD49dlo) T cells

Splenocytes from lymphoreplete, unmanipulated mice were analyzed for basal mRNA levels. We hypothesized, based on previous data from our lab and others, that many cytokine/inflammatory response genes would show an increase from naive CD5lo<CD5hi<Virtual memory. mRNA was analyzed from mouse splenocytes separated into naive CD5lo, naive CD5hi, and virtual memory cells. Mice were lymphoreplete and unmanipulated.