Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency

microscopy data involved in the ariticle "Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency". Our work reveals a new origin selection mechanism that the replication efficiency of origins is determined by their physical distribution in the chromatin domain, which undergoes a transcription-dependent structural reorganization process. Our model explains the complex links between replication origin efficiency and many genetic and epigenetic signatures that mark active transcription. The coordination between DNA replication, transcription, and chromatin organization inside individual TADs also provides new insights into the biological functions of sub-domain chromatin structural dynamics.