Transcriptome-wide identification of nuclear and cytoplasmic RNA-binding sites of FUS RBD-only constructs. Transcriptome-wide identification of nuclear and cytoplasmic RNA-binding sites of FUS RBD-only constructs

We report the binding sites of three FUS constructs: (1) The full-length protein (FL); (2) A deletion mutant consisting of amino acids 242-526 (CT); and (3) A aa 242-526 deletion mutant with ALS-associated P525L mutation and heterologous nuclear export signal (CTstar) with the aim to assess the role of protein-protein interactions on RNA-binding and to identify nuclear and cytoplasmic RNA-binding sites of Fused in Sarcoma (FUS). Overall design: Three HITS-CLIP experiments were performed in triplicate each. Input RNA was sequenced after depletion of ribosomal RNA.