RNA-seq of THESC in SRF-deficient vs Normal Conditions

The transcription factor SRF plays known roles in mitogenic cell response and muscle cell development, and potential roles in endometrial stromal decidualization have been reported. After RNAi-based SRF knockdown in hTERT-immortalized human endometrial stromal cells (THESC), SRF-deficient cells exhibited disrupted cytoskeletal remodeling, cell cycle, and extracellular matrix genes. These data show that SRF is important for endometrial stromal cell homeostasis. Overall design: THESCs (American Type Culture Collection #CRL-4003) were treated with control non-targeting siRNA or siSRF in triplicate followed by RNA-seq to analyze the impact of SRF deficiency on the transcriptome.