Gut microbiota composition associate with the capacity of human peripheral blood monocytes to differentiate into immunogenic dendritic cells in vitro

Although promising for active immunization in cancer patients, dendritic cells (DCs) vaccines generated in vitro display high inter-individual variability in their immunogenicity, which mostly limits their therapeutic efficacy. Gut microbiota composition is key emerging factor affecting individuals' immune responses, but it is unknown how it affects the variability of donors' precursor cells to differentiate into immunogenic DCs in vitro. By analyzing gut microbiota composition in 14 healthy donors, along with the phenotype and cytokines production by monocyte derived DCs, we found significant correlations between immunogenic properties of DC and microbiota composition.